Singla Lab IIT Roorkee

Research

What the lab works on

We build computational frameworks that sit between biology, data science and engineering. Three threads run through the lab right now — cellular architecture from tomography, AI for point-of-care diagnostics, and language models for Sanskrit.

तत्कर्म यन्न बन्धाय सा विद्या या विमुक्तये।
आयासायापरं कर्म विद्यान्या शिल्पनैपुणम्॥

tat karma yan na bandhāya sā vidyā yā vimuktaye | āyāsāyāparaṁ karma vidyānyā śilpanaipuṇam ||

That is work which does not bind; that is knowledge which sets free. Other work is only labour, other knowledge only craft. — Viṣṇu Purāṇa 1.19.41

01  —  Cellular architecture

Mitochondrial network modelling with soft X-ray tomography

Soft X-ray tomography (SXT) rapidly maps ultrastructure across whole, intact cells. We build the tools that turn those volumes into measurable biology.

SXT is an emerging technique for rapidly mapping ultrastructure in whole cells without fixation or sectioning. The difficulty is downstream: a tomogram is a dense grey volume, and the biology only appears once organelles are segmented, individuated and measured.

Our group develops robust segmentation and morphological profiling pipelines for mitochondria in these volumes, and uses them to ask how network structure, density and subcellular location are coupled — and how that coupling shifts under drug treatment.

The work is carried out with collaborators at USC and UCSF, and has appeared in Science Advances, Structure and the Journal of Structural Biology.

Soft X-ray tomographySegmentationMorphometryβ-cells
N 2.4 µm 2 µm density × location distance from nucleus density I II III
Cellular architecture

02  —  AI for healthcare

Non-invasive diagnostics from images and signals

Jaundice affects 60–80% of newborns worldwide. A phone camera should be enough to triage it.

Neonatal jaundice occurs in 60–80% of neonates worldwide and is one of the most common morbidities in both term and preterm neonates. Where a laboratory bilirubin assay is hours or kilometres away, early detection fails for logistical reasons rather than clinical ones.

We are building smartphone-based, non-invasive bilirubin estimation trained on Indian cohorts, in partnership with PGIMER Chandigarh — explicitly targeting skin-tone distributions and imaging conditions that existing models were not built for.

A parallel thread works on ECG: patient-independent reconstruction of missing leads using pathology-aware multi-view contrastive learning, so that a reduced-lead recording can still support diagnosis.

Neonatal jaundiceECG reconstructionContrastive learningPoint-of-care
images · neonatal bilirubin TcB est. 14.2 mg/dL fitted on cohort skin tones signals · ECG lead reconstruction recorded reconstructed 3 → 12 leads
AI for healthcare

03  —  Low-resource NLP

AI models for Sanskrit

Sanskrit is morphologically rich and data-poor — exactly the regime where modern NLP is weakest.

Sanskrit combines dense morphology, free word order and sandhi with a very small parallel corpus. That makes it a genuine stress test for translation methods rather than a benchmark to be saturated.

We have released Sāmayik, a benchmark and dataset for English–Sanskrit translation, and Samasamayik, a parallel dataset for Hindi–Sanskrit — and we develop training methods such as reconstruction-anchored semi-supervised training built for the low-resource regime.

The work is carried out with collaborators at IIT Delhi and IIT Roorkee's Centre for Indian Knowledge System.

Machine translationLow-resource NLPDatasetsIndian Knowledge Systems
वाक्यम् translate a sentence reconstruct वाक्यम् anchor Sāmayik · Samasamayik · RECAST
Low-resource NLP
Earlier threads 2

Lines of work the lab has taken as far as it means to for now. The methods and the papers stand; we are simply not adding to them this year.

Visual proteomics

Template-free macromolecular discovery in cryo-electron tomograms

Cryo-ET resolves cellular structure at molecular resolution. We look for the complexes nobody knew to search for.

Cryo-electron tomography images the cell at molecular resolution, but extracting information about macromolecular complexes from a cellular tomogram is a systematic problem, not a per-particle one. Conventional template matching can only find what you already have a reference for.

We develop frameworks for template-free, unsupervised discovery of distinct complexes from large, heterogeneous particle sets — including scoring functions that rank the quality of 3D subtomogram clusters, and de novo structural pattern mining across whole tomograms.

This line of work is pursued in collaboration with researchers at UCLA and has been highlighted in Nature Methods as template-free visual proteomics.

Cryo-ETSubtomogram averagingUnsupervised learningPattern mining
no templates, no reference cluster quality .84 .71 .53 .22 ranked, not thresholded
Visual proteomics

World in a Cell

A visual design language for biological structure

If you cannot draw the cell, you cannot reason about it. We designed a grammar for drawing it.

World in a Cell is an immersive experience built around a model of the pancreatic beta cell. Representing tens of thousands of molecular species inside a real-time environment demanded a representation that was both scientifically honest and cheap to render.

We developed a new visual design language in which tetrahedral building blocks compose into molecules and organelles, giving a consistent visual grammar across scales — from a single protein to the whole cellular environment — while remaining efficient to animate and interact with.

The language was published in Structure and underpins the ongoing artscience collaboration with the World Building Media Lab at USC.

Scientific visualizationImmersive mediaDesign languageBeta cell
one primitive, every scale 4.8 Å helix insulin hexamer amino acid
World in a Cell

Work with us

We look for people who want to build, question and grow — not collect credentials.

See open routes